N-acetyl-D-neuraminic acid lyase generates the sialic acid for colominic acid biosynthesis in Escherichia coli K1.

  title={N-acetyl-D-neuraminic acid lyase generates the sialic acid for colominic acid biosynthesis in Escherichia coli K1.},
  author={M. A. Ferrero and {\'A}. Reglero and M. Fernandez-Lopez and R. Ordas and L. Rodr{\'i}guez-Aparicio},
  journal={The Biochemical journal},
  volume={317 ( Pt 1)},
Colominic acid is a capsular homopolymer from Escherichia coli K1 composed of alpha (2-8)-linked N-acetyl-D-neuraminic acid (NeuAc) residues. Recently, we have described that NeuAc synthesis in this bacterium occurs through the action of NeuAc lyase (EC [ Rodríguez-Aparicio, Ferrero and Reglero (1995) Biochem. J.308, 501-505]. In the present work we analysed and characterized this enzyme. E. coli K1 NeuAc lyase is detected from the early logarithmic phase of growth, is induced by NeuAc… Expand
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Recombinant DNA methodology provides a powerful tool for studying whether the K1 antigen is sufficient to confer virulence or if other E. coli structures are necessary, and cloned K1 genes synthesize a capsule in E.E. coli K12 indistinguishable chemically and immunologically from that of wild-type K1 strains. Expand