N-Glycan Modification of a Recombinant Protein via Coexpression of Human Glycosyltransferases in Silkworm Pupae

@inproceedings{Kato2017NGlycanMO,
  title={N-Glycan Modification of a Recombinant Protein via Coexpression of Human Glycosyltransferases in Silkworm Pupae},
  author={Tatsuya Kato and Natsumi Kako and Kotaro Kikuta and Takatsugu Miyazaki and Sachiko Kondo and Hirokazu Yagi and Koichi Kato and Enoch Y. Park},
  booktitle={Scientific Reports},
  year={2017}
}
Recombinant proteins produced in insect cells and insects, unlike those produced in mammalian cells, have pauci-mannose-type N-glycans. In this study, we examined complex-type N-glycans on recombinant proteins via coexpression of human β-1,2-N-acetylglucosaminyltransferase II (hGnT II) and human β1,4-galactosyltransferase (hGalT I) in silkworm pupae, by using the Bombyx mori nucleopolyhedrovirus (BmNPV) bacmid system. The actin A3 promoter from B. mori and the polyhedrin promoter from… CONTINUE READING
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