N-Alkylated 6'-aminoluciferins are bioluminescent substrates for Ultra-Glo and QuantiLum luciferase: new potential scaffolds for bioluminescent assays.

@article{Woodroofe2008NAlkylated6A,
  title={N-Alkylated 6'-aminoluciferins are bioluminescent substrates for Ultra-Glo and QuantiLum luciferase: new potential scaffolds for bioluminescent assays.},
  author={C. Woodroofe and John W Shultz and M. G. Wood and Jean Osterman and J. Cali and W. Daily and P. Meisenheimer and D. Klaubert},
  journal={Biochemistry},
  year={2008},
  volume={47 39},
  pages={
          10383-93
        }
}
  • C. Woodroofe, John W Shultz, +5 authors D. Klaubert
  • Published 2008
  • Chemistry, Medicine
  • Biochemistry
  • A set of 6'-alkylated aminoluciferins are shown to be bioluminescent substrates for Ultra-Glo and QuantiLum luciferases. These studies demonstrate that both the engineered and wild-type firefly luciferases tolerate much greater steric bulk at the 6' position of luciferin than has been previously reported. The nature of the alkyl substituent strongly affects the strength of the bioluminescent signal, which varies widely based on size, shape, and charge. Several compounds were observed to… CONTINUE READING

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    References

    Publications referenced by this paper.
    SHOWING 1-10 OF 23 REFERENCES
    Homogeneous, Bioluminescent Protease Assays: Caspase-3 as a Model
    • 98
    New bioluminogenic substrates for monoamine oxidase assays.
    • 65
    Structural basis for the spectral difference in luciferase bioluminescence
    • 404
    • Highly Influential
    Use of firefly luciferase in ATP-related assays of biomass, enzymes, and metabolites.
    • 222
    Stereo-specificity and firefly bioluminescence, a comparison of natural and synthetic luciferins.
    • 63
    • PDF
    Luminogenic cytochrome P450 assays
    • 128
    Firefly luciferase gene: structure and expression in mammalian cells.
    • 2,520
    • PDF
    Halohydrin and oxime derivatives of radicicol: synthesis and antitumor activities.
    • 88