N-Alkylated 6'-aminoluciferins are bioluminescent substrates for Ultra-Glo and QuantiLum luciferase: new potential scaffolds for bioluminescent assays.

@article{Woodroofe2008NAlkylated6A,
  title={N-Alkylated 6'-aminoluciferins are bioluminescent substrates for Ultra-Glo and QuantiLum luciferase: new potential scaffolds for bioluminescent assays.},
  author={Carolyn C. Woodroofe and John W Shultz and Monika G. Wood and Jean Osterman and James J. Cali and William J R Daily and Poncho L. Meisenheimer and Dieter H. Klaubert},
  journal={Biochemistry},
  year={2008},
  volume={47 39},
  pages={10383-93}
}
A set of 6'-alkylated aminoluciferins are shown to be bioluminescent substrates for Ultra-Glo and QuantiLum luciferases. These studies demonstrate that both the engineered and wild-type firefly luciferases tolerate much greater steric bulk at the 6' position of luciferin than has been previously reported. The nature of the alkyl substituent strongly affects the strength of the bioluminescent signal, which varies widely based on size, shape, and charge. Several compounds were observed to… CONTINUE READING

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