Myosin phosphorylation in intact platelets.

  title={Myosin phosphorylation in intact platelets.},
  author={James L. Daniel and I R Molish and Holm Holmsen},
  journal={The Journal of biological chemistry},
  volume={256 14},
The phosphorylation state of myosin in intact platelets has been investigated with alkaline urea-polyacrylamide gel electrophoresis. In gels of control cells, a band was found that co-migrated with the dephosphorylated form of isolated platelet myosin 20,000-dalton light chain. Stimulation of the cells by thrombin produced a dose-dependent shift of this band to the same position as that of the phosphorylated light chain. Conversion to the phosphorylated position was both complete and saturable… CONTINUE READING
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