Myosin phosphatase-targeting subunit 1 controls chromatid segregation.

  title={Myosin phosphatase-targeting subunit 1 controls chromatid segregation.},
  author={Fumio Matsumura and Yoshihiko Yamakita and Shigeko Yamashiro},
  journal={The Journal of biological chemistry},
  volume={286 12},
Myosin phosphatase is a heterotrimeric holoenzyme consisting of myosin phosphatase-targeting subunit 1 (MYPT1), a catalytic subunit of PP1Cβ, and a 20-kDa subunit of an unknown function. We have previously reported that myosin phosphatase also controls mitosis, apparently by antagonizing polo-like kinase 1 (PLK1). Here we found that depletion of MYPT1 by siRNA led to precocious chromatid segregation when HeLa cells were arrested at metaphase by a proteasome inhibitor, MG132, or by Cdc20… CONTINUE READING