Myosin light chain kinase binding to actin filaments.

  title={Myosin light chain kinase binding to actin filaments.},
  author={Lula Smith and James T Stull},
  journal={FEBS letters},
  volume={480 2-3},
Smooth muscle myosin light chain kinase (MLCK) plays important roles in contractile-motile processes of a variety of cells. Three DFRxxL motifs at the kinase N-terminus (residues 2-63) are critical for high-affinity binding to actin-containing filaments [Smith et al. (1999) J. Biol. Chem. 274, 29433-29438]. A GST fusion protein containing residues 1-75 of MLCK (GST75-MLCK) bound maximally to both smooth muscle myofilaments and F-actin at 0.28 and 0.31 mol GST75-MLCK/mol actin with respective K… CONTINUE READING