Myosin VI steps via a hand-over-hand mechanism with its lever arm undergoing fluctuations when attached to actin.

@article{Yildiz2004MyosinVS,
  title={Myosin VI steps via a hand-over-hand mechanism with its lever arm undergoing fluctuations when attached to actin.},
  author={Ahmet Yildiz and Hyokeun Park and Dan Safer and Zhao-Hui Yang and Li-qiong Chen and Paul R Selvin and H Lee Sweeney},
  journal={The Journal of biological chemistry},
  year={2004},
  volume={279 36},
  pages={37223-6}
}
Myosin VI is a reverse direction myosin motor that, as a dimer, moves processively on actin with an average center-of-mass movement of approximately 30 nm for each step. We labeled myosin VI with a single fluorophore on either its motor domain or on the distal of two calmodulins (CaMs) located on its putative lever arm. Using a technique called FIONA (fluorescence imaging with one nanometer accuracy), step size was observed with a standard deviation of <1.5 nm, with 0.5-s temporal resolution… CONTINUE READING
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