Myosin-II inhibition and soft 2D matrix maximize multinucleation and cellular projections typical of platelet-producing megakaryocytes.

@article{Shin2011MyosinIIIA,
  title={Myosin-II inhibition and soft 2D matrix maximize multinucleation and cellular projections typical of platelet-producing megakaryocytes.},
  author={Jeong Seob Shin and James H. Swift and Kyle Richard Spinler and Dennis E Discher},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={2011},
  volume={108 28},
  pages={
          11458-63
        }
}
Cell division, membrane rigidity, and strong adhesion to a rigid matrix are all promoted by myosin-II, and so multinucleated cells with distended membranes--typical of megakaryocytes (MKs)--seem predictable for low myosin activity in cells on soft matrices. Paradoxically, myosin mutations lead to defects in MKs and platelets. Here, reversible inhibition of myosin-II is sustained over several cell cycles to produce 3- to 10-fold increases in polyploid MK and a number of other cell types. Even… CONTINUE READING

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Disruption of the Dictyostelium myosin heavy chain gene by homologous recombination

  • A De Lozanne, JA Spudich
  • Science
  • 1987
Highly Influential
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