Myoplasmic free Mg2+ concentration during repetitive stimulation of single fibres from mouse skeletal muscle.

@article{Westerblad1992MyoplasmicFM,
  title={Myoplasmic free Mg2+ concentration during repetitive stimulation of single fibres from mouse skeletal muscle.},
  author={H{\aa}kan Westerblad and David G. Allen},
  journal={The Journal of physiology},
  year={1992},
  volume={453},
  pages={413-34}
}
1. The role of the myoplasmic free Mg2+ concentration ([Mg2+]i) in fatigue was studied in intact single fibres isolated from mouse skeletal muscle. Fatigue was produced by repeated tetanic stimulation. The fluorescent Mg2+ indicator furaptra was pressure injected into fibres. In vivo calibrations were performed to convert fluorescence signals into [Mg2+]i. 2. [Mg2+]i at rest was 0.78 +/- 0.05 mM (mean +/- S.E.M., n = 14). An increase of the extracellular [Mg2+] from 0.5 to 20 mM resulted in a… CONTINUE READING

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