Myoplasmic calcium transients in intact frog skeletal muscle fibers monitored with the fluorescent indicator furaptra

@article{Konishi1991MyoplasmicCT,
  title={Myoplasmic calcium transients in intact frog skeletal muscle fibers monitored with the fluorescent indicator furaptra},
  author={Masato Konishi and Stephen Hollingworth and Amy B. Harkins and Stephen M Baylor},
  journal={The Journal of General Physiology},
  year={1991},
  volume={97},
  pages={271 - 301}
}
Furaptra (Raju, B., E. Murphy, L. A. Levy, R. D. Hall, and R. E. London. 1989. Am. J. Physiol. 256:C540-C548) is a "tri-carboxylate" fluorescent indicator with a chromophore group similar to that of fura-2 (Grynkiewicz, G., M. Poenie, and R. Y. Tsien. 1985. J. Biol. Chem. 260:3440-3450). In vitro calibrations indicate that furaptra reacts with Ca2+ and Mg2+ with 1:1 stoichiometry, with dissociation constants of 44 microM and 5.3 mM, respectively (16-17 degrees C; ionic strength, 0.15 M; pH, 7.0… CONTINUE READING

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Effects of external calcium concentration and pH on charge movement in frog skeletal muscle

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