Myofibroblasts from scleroderma skin synthesize elevated levels of collagen and tissue inhibitor of metalloproteinase (TIMP-1) with two forms of TIMP-1.

@article{Kirk1995MyofibroblastsFS,
  title={Myofibroblasts from scleroderma skin synthesize elevated levels of collagen and tissue inhibitor of metalloproteinase (TIMP-1) with two forms of TIMP-1.},
  author={T Z Kirk and Michelle E Mark and Chu Chang Chua and Balvin H. L. Chua and Maureen D. Mayes},
  journal={The Journal of biological chemistry},
  year={1995},
  volume={270 7},
  pages={3423-8}
}
Cultured fibroblasts derived from skin biopsies from scleroderma patients and normal individuals were examined for the presence of smooth muscle alpha-actin, a marker for myofibroblasts. Six of eight scleroderma cell lines were found to be 50% or more positive for alpha-actin while three of four normal lines and one cell line derived from unaffected skin of a scleroderma patient were less than 10% positive. The cultured fibroblasts from affected scleroderma skin were largely myofibroblasts, a… CONTINUE READING