Myo-inositol facilitators IolT1 and IolT2 enhance D-mannitol formation from D-fructose in Corynebacterium glutamicum.

@article{Bumchen2009MyoinositolFI,
  title={Myo-inositol facilitators IolT1 and IolT2 enhance D-mannitol formation from D-fructose in Corynebacterium glutamicum.},
  author={Carsten B{\"a}umchen and Eva Krings and Stephanie Bringer and Lothar Eggeling and Hermann 4 Sahm},
  journal={FEMS microbiology letters},
  year={2009},
  volume={290 2},
  pages={227-35}
}
Reduction of D-fructose to D-mannitol by whole-cell biotransformation with recombinant resting cells of Corynebacterium glutamicum ATCC13032 requires the coexpression of mdh and fdh, which encode mannitol and formate dehydrogenases, respectively. However, d-mannitol formation is limited by the uptake of d-fructose in its unphosphorylated form, because additional expression of the sugar facilitator from Zymomonas mobilis resulted in a significantly increased productivity. Here we identified… CONTINUE READING