Myeloablation-associated deletion of ORF4 in a human coronavirus 229E infection

  title={Myeloablation-associated deletion of ORF4 in a human coronavirus 229E infection},
  author={Alexander L Greninger and Gregory Pepper and Ryan C. Shean and Anne Cent and Isabel Palileo and Jane Kuypers and Joshua T. Schiffer and Keith R. Jerome},
  booktitle={npj Genomic Medicine},
We describe metagenomic next-generation sequencing (mNGS) of a human coronavirus 229E from a patient with AML and persistent upper respiratory symptoms, who underwent hematopoietic cell transplantation (HCT). mNGS revealed a 548-nucleotide deletion, which comprised the near entirety of the ORF4 gene, and no minor allele variants were detected to suggest a mixed infection. As part of her pre-HCT conditioning regimen, the patient received myeloablative treatment with cyclophosphamide and 12 Gy… CONTINUE READING