Mycothiol protects Corynebacterium glutamicum against acid stress via maintaining intracellular pH homeostasis, scavenging ROS, and S-mycothiolating MetE.

@article{Liu2016MycothiolPC,
  title={Mycothiol protects Corynebacterium glutamicum against acid stress via maintaining intracellular pH homeostasis, scavenging ROS, and S-mycothiolating MetE.},
  author={Yingbao Liu and Xiaobing Yang and Yajie Yin and Jinshui Lin and Can Chen and Junfeng Pan and Meiru Si and Xihui Shen},
  journal={The Journal of general and applied microbiology},
  year={2016},
  volume={62 3},
  pages={
          144-53
        }
}
Mycothiol (MSH) plays a major role in protecting cells against oxidative stress and detoxification from a broad range of exogenous toxic agents. In the present study, we reveal that intracellular MSH contributes significantly to the adaptation to acidic conditions in the model organism Corynebacterium glutamicum. We present evidence that MSH confers C. glutamicum with the ability to adapt to acidic conditions by maintaining pHi homeostasis, scavenging reactive oxygen species (ROS), and… 
Impaired oxidative stress and sulfur assimilation contribute to acid tolerance of Corynebacterium glutamicum
TLDR
Physiological characterizations revealed that the adapted strain achieved improved cell viability after acid-stress challenge, with a higher cytoplasmic pHin level, a lower intracellular reactive oxygen species (ROS), and an enhanced morphological integrity of the cells, when compared to those of the original control strain.
The Industrial Organism Corynebacterium glutamicum Requires Mycothiol as Antioxidant to Resist Against Oxidative Stress in Bioreactor Cultivations
TLDR
Bioreactor experiments with C. glutamicum expressing the Mrx1-roGFP2 redox biosensor revealed a strong oxidative shift in the MSH redox potential (EMSH) at pO2 values above 20%.
The Industrial Organism Corynebacterium glutamicum Requires Mycothiol as Antioxidant to Resist against Oxidative Stress in Bioreactor Cultivations
In aerobic environments, bacteria are exposed to reactive oxygen species (ROS). To avoid an excess of ROS, microorganisms are equipped with powerful enzymatic and non-enzymatic antioxidants.
Function of alkyl hydroperoxidase AhpD in resistance to oxidative stress in Corynebacterium glutamicum.
TLDR
It is reported that C. glutamicum AhpDs were disulfide oxidoreductases behaving like thioredoxin (Trx) in regenerating thiol-dependent peroxidase for stress response, which provides the theoretical basis for an in-depth study of the reduction system in ahpC-lacking bacteria.
Chemistry and Redox Biology of Mycothiol.
TLDR
The description of MSH/Mrx-1-dependent activities of peroxidases helped to explain the higher susceptibility to oxidants observed in Actinomycetes lacking MSH, and the first mycothiol-S-transferase, member of the DinB superfamily of proteins, was described.
Recent advances of pH homeostasis mechanisms in Corynebacterium glutamicum.
TLDR
This review summarizes the current knowledge of pH homeostasis mechanisms adopted by C. glutamicum for coping with low acidic pH and high alkaline pH stresses, and discusses the general strategies and prospects on metabolic engineering of C.glutamicum to improve alkaline or acid resistance.
Chemistry and Redox Biology of Mycothiol.
Abstract Significance: Mycothiol (MSH, AcCys-GlcN-Ins) is the main low-molecular weight (LMW) thiol of most Actinomycetes, including the human pathogen Mycobacterium tuberculosis that affects
The glyceraldehyde-3-phosphate dehydrogenase GapDH of Corynebacterium diphtheriae is redox-controlled by protein S-mycothiolation under oxidative stress
TLDR
It is shown here that S-mycothiolation can function in redox-regulation and protection of the GapDH active site against overoxidation in C. diphtheriae which can be reversed by both, the Mrx1 and Trx pathways.
Functional comparison of methionine sulphoxide reductase A and B in Corynebacterium glutamicum.
TLDR
The msrB gene showed a slight effect on utilizing methionine sulfoxide (MetO) as the sole Met source; however, the survival rates showed no sensitivity to oxidants and it could reduce MetO to Met by the Trx/TrxR reducing system, which is useful for expanding the understanding of the functions of Msr in this important industrial microbe.
Role of ClpB From Corynebacterium crenatum in Thermal Stress and Arginine Fermentation
TLDR
The results showed that DnaK/ClpB is not essential for the survival of C. crenatum MT under pH and alcohol stresses and demonstrated that deletion of the clpB gene affected glucose consumption and L-arginine, L-glutamate, and lactate production during fermentation.
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