The vaginal secretion of 194 women was subjected to bacteriological examination. The results of cultivation examinations were compared with MOP examinations. The widest bacterial spectrum was recorded in MOP II. No differences were recorded as regards detection of lactobacilli and potentially pathogenic bacteria in MOP I, II and III. The percentage of women with Ureaplasma urealyticum was equal in case of MOP I, II and III. Mycoplasma hominis was isolated significantly more frequently in MOP II than in MOP III. As compared with results of examinations in asymptomatic women, those with colpitis were significantly less frequently colonized with Ureaplasma urealyticum and Mycoplasma hominis. The authors did not record any differences in the composition of the microbial flora in women colonized with mycoplasmas and those without mycoplasmas.