Mycobacterium smegmatis DNA gyrase: cloning and overexpression in Escherichia coli.

@article{Madhusudan1995MycobacteriumSD,
  title={Mycobacterium smegmatis DNA gyrase: cloning and overexpression in Escherichia coli.},
  author={Kuncha Madhusudan and Valakunja Nagaraja},
  journal={Microbiology},
  year={1995},
  volume={141 ( Pt 12)},
  pages={3029-37}
}
The cloning and characterization of DNA gyrase genes from Mycobacterium smegmatis is described. The DNA sequence of 5119 bp encoding both gyrB and gyrA genes was determined. The gene gyrB precedes gyrA with a short intergenic region of 29 nucleotides. The proteins encoded, GyrB and GyrA, exhibit 45-80% identity to gyrase polypeptides from other bacteria. The genes were further engineered for overexpression in Escherichia coli. Both genes were individually cloned into a phage T7 expression… CONTINUE READING