Mycobacterial cells have dual nickel-cobalt sensors: sequence relationships and metal sites of metal-responsive repressors are not congruent.

@article{Campbell2007MycobacterialCH,
  title={Mycobacterial cells have dual nickel-cobalt sensors: sequence relationships and metal sites of metal-responsive repressors are not congruent.},
  author={Duncan Russell Campbell and Kaye E. Chapman and Kevin J Waldron and Stephen Tottey and Sharon L. Kendall and Gabriele Cavallaro and Claudia Andreini and Jason Hinds and Neil G. Stoker and Nigel J. Robinson and Jennifer S. Cavet},
  journal={The Journal of biological chemistry},
  year={2007},
  volume={282 44},
  pages={
          32298-310
        }
}
A novel ArsR-SmtB family transcriptional repressor, KmtR, has been characterized from mycobacteria. Mutants of Mycobacterium tuberculosis lacking kmtR show elevated expression of Rv2025c encoding a deduced CDF-family metal exporter. KmtR-dependent repression of the cdf and kmtR operator-promoters was alleviated by nickel and cobalt in minimal medium. Electrophoretic mobility shift assays and fluorescence anisotropy show binding of purified KmtR to nucleotide sequences containing a region of… CONTINUE READING
28 Citations
0 References
Similar Papers

Citations

Publications citing this paper.
Showing 1-10 of 28 extracted citations

Similar Papers

Loading similar papers…