Mycobacterial RNA polymerase forms unstable open promoter complexes that are stabilized by CarD

@inproceedings{Davis2015MycobacterialRP,
  title={Mycobacterial RNA polymerase forms unstable open promoter complexes that are stabilized by CarD},
  author={Elizabeth S Davis and James Chen and Katherine Kelly Leon and Seth A Darst and Elizabeth A. Campbell},
  booktitle={Nucleic acids research},
  year={2015}
}
Escherichia coli has served as the archetypal organism on which the overwhelming majority of biochemical characterizations of bacterial RNA polymerase (RNAP) have been focused; the properties of E. coli RNAP have been accepted as generally representative for all bacterial RNAPs. Here, we directly compare the initiation properties of a mycobacterial transcription system with E. coli RNAP on two different promoters. The detailed characterizations include abortive transcription assays, RNAP… CONTINUE READING