Corpus ID: 88479056

Mycobacterial Haloalkane Dehalogenases: Cloning, BiochemicalProperties and distribution.

  title={Mycobacterial Haloalkane Dehalogenases: Cloning, BiochemicalProperties and distribution.},
  author={A. Jesenská and M. Pavlová and M. Strouhal and R. Chaloupkova and Iva Tě{\vs}{\'i}nsk{\'a} and Marta Monincov{\'a} and Z. Prokop and M. Barto{\vs} and I. Pavlík and I. Rychlik and P. Mobius and Y. Nagata and J. Damborsk{\'y}},
  journal={Applied and Environmental Microbiology},
Haloalkane dehalogenases are enzymes catalysing the cleavage of the carbon-halogen bond by a hydrolytic mechanism. Genomes of Mycobacterium tuberculosis and M. bovis contain at least two open reading frames coding for the polypeptides showing a high sequence similarity with biochemically characterised haloalkane dehalogenases. In this paper we have described the cloning of the haloalkane dehalogenase genes dmbA and dmbB from M. bovis 5033/66 and proved the dehalogenase activity of their… Expand
The identification of catalytic pentad in the haloalkane dehalogenase DhmA from Mycobacterium avium N85: reaction mechanism and molecular evolution.
Haloalkane dehalogenase DhmA from Mycobacterium avium N85 showed poor expression and low stability when produced in Escherichia coli. Here, we present expression DhmA in newly constructed pK4RPExpand
Structure-Function Relationships and Engineering of Haloalkane Dehalogenases
The structure–function relationships for haloalkane dehalogenases, representing one of the best characterized families of enzymes involved in degradation of halogenated compounds is described. AExpand
Stereoselectivity and conformational stability of haloalkane dehalogenase DbjA from Bradyrhizobium japonicum USDA110: the effect of pH and temperature
Unique catalytic activity and structural stability of DbjA in a broad pH range, combined with high enantioselectivity with particular substrates, make this enzyme a very versatile biocatalyst. Expand
Biochemical Characterization of Haloalkane Dehalogenases DrbA and DmbC, Representatives of a Novel Subfamily
ABSTRACT This study focuses on two representatives of experimentally uncharacterized haloalkane dehalogenases from the subfamily HLD-III. We report biochemical characterization of the expressionExpand
Bacterial strain Bradyrhizobium japonicum USDA 110 produces DbjA dehalogenase, which degrades halogenated aliphatic pollutants and displays wide substrate specificity towards 1,2 – dibromoethane. Expand