Mycobacteria: Bugs and bugbears (Two steps forward and one step back)

  title={Mycobacteria: Bugs and bugbears (Two steps forward and one step back)},
  author={Tanya Parish and Neil G. Stoker},
  journal={Molecular Biotechnology},
The use of molecular techniques to study the mycobacteria has advanced greatly since the first genomic libraries of Mycobacterium tuberculosis and M. leprae were constructed in 1985. However, there are still pitfalls for the unwary. Most of the problems associated with the use of molecular techniques to study mycobacteria can be related to one of the following problems: slow growth rate causing problems with contamination; the formation of macroscopic clumps when grown in culture; resistance to… 

Targeting the histidine pathway in Mycobacterium tuberculosis.

A comprehensive overview of Mycobacterium tuberculosis histidine pathway enzymes as attractive targets for the development of new antimycobacterial agents is provided, mainly summarizing the previously reported inhibition data for Mtb or orthologous proteins.

Computational exploration of transmission and acquisition of drug-resistant tuberculosis

This research explored the transmission and acquisition of drug-resistant tuberculosis, and used modeling and algorithms in support of treatment and control of tuberculosis.

Reduction of Human DNA Contamination in Clinical Cerebrospinal Fluid Specimens Improves the Sensitivity of Metagenomic Next-Generation Sequencing

The results demonstrate that the use of mNGS of centrifuged supernatants from clinical CSF samples in patients with TBM and CM is a simple and effective method to improve the sensitivity of pathogen detection.

Biochemical, Biophysical, and Structural Characterisation of Glutamate Racemase from Mycobacterium tuberculosis and Mycobacterium smegmatis

Structural analysis of their active sites in comparison to that of other glutamate racemase structures reveals a notable feature in mycobacterial MurI active site architecture, and based on this feature, a possible drug design targeting the MurIMtb active site has been suggested.

Molecular Diagnosis, Detection and Treatment of Drug Resistant Mycobacterium tuberculosis

Pulmonary tuberculosis mainly results from reactivation of a tuberculous f ocus after hematogenous dissemination or lymphogenous spread from a primary, usually pulmonary focus, and may mimick other pathologies.

Homology modeling, molecular dynamics and inhibitor binding study on MurD ligase of Mycobacterium tuberculosis

Few pharmacophoric features essential for Mtb-MurD ligase inhibitory activity are identified and which can further been utilized for the discovery of putative antitubercular chemotherapy.

Design, synthesis and in vivo/in vitro screening of novel chlorokojic acid derivatives

A series of novel Mannich bases of chlorokojic acid exhibit significant antitubercular activity in a MIC range of 4 to 32 µg/mL, especially compound 18 against M. avium.

To Find out the Essentiality of Rv0526 Gene in Virulence of Mycobacterium tuberculosis by using In silico Approaches

Tuberculosis has emerged as a major world health problem, with almost one-third of the world population today infected with Mycobacterium tuberculosis H37Rv (M. tuberculosis). This gram-positive

Microfluidics as a Novel Technique for Tuberculosis: From Diagnostics to Drug Discovery

The practices of microfluidics have promising future applications for diagnosing and treating TB and the use of this novel technique so far in M. tuberculosis diagnostics, research methods, and drug discovery platforms is discussed.



Isolation and characterization of efficient plasmid transformation mutants of Mycobacterium smegmatis

Efficient plasmid transformation of M. smegmatis will facilitate the analysis of mycobacterial gene function, expression and replication and thus aid in the development of BCG as a multivalent recombinant vaccine vector and in the genetic analysis of the virulence determinants of pathogenicMycobacteria.

The role of polymerase chain reaction in the diagnosis of mycobacterial infections

Improved and novel techniques are proving useful in the diagnosis of tuberculosis and the likely development of commercial test systems should result in the widespread and routine use of PCR in time, however, it must be remembered that the technique detects both live and dead bacteria and the precise clinical relevance of PCR results will need to be determined.

Efficient transposition in mycobacteria: construction of Mycobacterium smegmatis insertional mutant libraries

The Tn611 transposon was inserted into pCG63, a temperature-sensitive plasmid isolated from an Escherichia coli-mycobacterial shuttle vector which contains the pAL5000 and pUC18 replicons, to generate a large number of insertional mutations in Mycobacterium smegmatis.

The urease locus of Mycobacterium tuberculosis and its utilization for the demonstration of allelic exchange in Mycobacterium bovis bacillus Calmette-Guérin.

Homologous recombination will be an invaluable genetic tool for deciphering the mechanisms of tuberculosis pathogenesis, a disease that causes 3 x 10(6) deaths a year worldwide.

Isolation by genetic labeling of a new mycobacterial plasmid, pJAZ38, from Mycobacterium fortuitum

Experiments showed pJAZ38 to be stably inherited in the absence of selection pressure and compatible with the most commonly used mycobacterial replicon, pAL5000, making it a useful tool for myCobacterial genetics.

Targeted replacement of the mycocerosic acid synthase gene in Mycobacterium bovis BCG produces a mutant that lacks mycosides.

Thin-layer and radio-gas chromatographic analyses of the lipids derived from [1-14C]propionate showed that the mutant was incapable of synthesizing mycocerosic acids and mycosides, and homologous recombination with double crossover was achieved in a slow-growing mycobacterium with an intron-containing RecA.

Deciphering the biology of Mycobacterium tuberculosis from the complete genome sequence

The complete genome sequence of the best-characterized strain of Mycobacterium tuberculosis, H37Rv, has been determined and analysed in order to improve the understanding of the biology of this slow-growing pathogen and to help the conception of new prophylactic and therapeutic interventions.

A mutant of Mycobacterium smegmatis defective in the biosynthesis of mycolic acids accumulates meromycolates.

  • J. LiuH. Nikaido
  • Biology, Chemistry
    Proceedings of the National Academy of Sciences of the United States of America
  • 1999
This work has isolated a mycolate-deficient mutant strain of Mycobacterium smegmatis mc2-155 by chemical mutagenesis followed by screening for increased sensitivity to novobiocin and found that this mutant was hypersensitive to other hydrophobic compounds such as crystal violet, rifampicin, and erythromycin.

Isolation and sequencing of the replication region of Mycobacterium avium plasmid pLR7

The Mycobacterium avium plasmid pLR7 capable of replication was identified and sequenced and an open reading frame encoding a putative Rep protein was identified.

Cloning of mycobacterial histidine synthesis genes by complementation of a Mycobacterium smegmatis auxotroph

Histidine‐requiring auxotrophs of Mycobacterium smegmatis were isolated following N‐methyl‐N′‐nitro‐ N‐nitrosoguanidine treatment, suggesting that the regulation of histidine biosynthesis in mycobacteria may differ from that of Escherichia coli.