The efficacy of silver synthesized biolarvicide with the help of entomopathogenic fungus, Beauveria bassiana, was assessed against the different larval instars of dengue vector, Aedes aegypti. The silver nanoparticles were observed and characterized by a scanning electron microscope (SEM) and energy-dispersive X-ray (EDX). A surface plasmon resonance band was observed at 420 nm in UV-vis spectrophotometer. The characterization was confirmed by shape (spherical), size 36.88–60.93 nm, and EDX spectral peak at 3 keV of silver nanoparticles. The synthesized silver nanoparticles have been tested against the different larval instars of Ae. aegypti at different concentrations for a period of 24 h. Ae. aegypti larvae were found more susceptible to the synthesized silver nanoparticles. The LC50 and LC90 values are 0.79 and 1.09 ppm with respect to the Ae. aegypti treated with B. bassiana (Bb) silver nanoparticles (AgNPs). First and second instar larvae of Ae. aegypti have shown cent percent mortality while third and fourth instars found 50.0, 56.6, 70.0, 80.0, and 86.6 and 52.4, 60.0, 68.5, 76.0, and 83.3 % mortality at 24 h of exposure in 0.06 and 1.00 ppm, respectively. It is suggested that the entomopathogenic fungus synthesized silver nanoparticles would be appropriate for environmentally safer and greener approach for new leeway in vector control strategy through a biological process.