Mutations that suppress the thermosensitivity of green fluorescent protein

  title={Mutations that suppress the thermosensitivity of green fluorescent protein},
  author={Kirby R. Siemering and Ralph Peter Golbik and Richard Sever and Jim Haseloff},
  journal={Current Biology},
BACKGROUND The green fluorescent protein (GFP) of the jellyfish Aequorea victoria has recently attracted great interest as the first example of a cloned reporter protein that is intrinsically fluorescent. Although successful in some organisms, heterologous expression of GFP has not always been straight forward. In particular, expression of GFP in cells that require incubation temperatures around 37 degrees C has been problematic. RESULTS We have carried out a screen for mutant forms of GFP… CONTINUE READING
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