Mutations that convert the four leucine codons of the Salmonella typhimurium leu leader to four threonine codons.

@article{Carter1985MutationsTC,
  title={Mutations that convert the four leucine codons of the Salmonella typhimurium leu leader to four threonine codons.},
  author={Philip W. Carter and Donald H. Weiss and Herbert Lee Weith and Jos{\'e} Mar{\'i}a P{\'e}rez Calvo},
  journal={Journal of bacteriology},
  year={1985},
  volume={162 3},
  pages={
          943-9
        }
}
In Salmonella typhimurium, expression of the leucine operon is regulated by a transcription attenuation mechanism. According to a current model of attenuation, elevated expression of this operon requires that a ribosome stall at one of four adjacent codons for leucine on a leader RNA. We used oligonucleotide-directed mutagenesis to convert the four leucine codons of the S. typhimurium leu leader to four threonine codons. Analysis of the resulting mutant operon showed that almost all regulation… CONTINUE READING

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