Mutations that alter the signal sequence of alkaline phosphatase in Escherichia coli.

@article{Michaelis1983MutationsTA,
  title={Mutations that alter the signal sequence of alkaline phosphatase in Escherichia coli.},
  author={Susan Michaelis and Hideyo Inouye and Donald S. Oliver and Jon et Beckwith},
  journal={Journal of bacteriology},
  year={1983},
  volume={154 1},
  pages={366-74}
}
A phoA-lacZ gene fusion was used to isolate mutants altered in the alkaline phosphatase signal sequence. This was done by selecting Lac+ mutants from a phoA-lacZ fusion strain that produces a membrane-bound hybrid protein and is unable to grow on lactose. Two such mutant derivatives were characterized. The mutations lie within the phoA portion of the fused gene and cause internalization of the hybrid protein. When the mutations were genetically recombined into an otherwise wild-type phoA gene… CONTINUE READING

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