Mutations that alter the pore function of the OmpF porin of Escherichia coli K12.

@article{Benson1988MutationsTA,
  title={Mutations that alter the pore function of the OmpF porin of Escherichia coli K12.},
  author={Sarah A. Benson and James L. Occi and B A Sampson},
  journal={Journal of molecular biology},
  year={1988},
  volume={203 4},
  pages={961-70}
}
We describe the isolation and characterization of mutations in ompF that alter the pore properties of the OmpF porin. The selection makes use of the fact that maltodextrins larger than maltotriose are too large to diffuse through the normal OmpF pore. By demanding growth on maltodextrins (Dex+) in the absence of the LamB protein, which is normally required for the uptake of these large sugars, we are able to obtain ompF mutations. These include transversions, transitions and small deletions. We… CONTINUE READING