Mutations on the N-terminal region abolish differentially the enzymatic activity, membrane-damaging activity and cytotoxicity of Taiwan cobra phospholipase A2.

@article{Chiou2008MutationsOT,
  title={Mutations on the N-terminal region abolish differentially the enzymatic activity, membrane-damaging activity and cytotoxicity of Taiwan cobra phospholipase A2.},
  author={Yi-Ling Chiou and Yun-Ching Cheng and Pei-Hsiu Kao and Jeh-Jeng Wang and Long-sen Chang},
  journal={Toxicon : official journal of the International Society on Toxinology},
  year={2008},
  volume={51 2},
  pages={270-9}
}
To examine the functional contribution of the N-terminal region to the activities of Naja naja atra phospholipase A(2) (PLA(2)), studies on three N-terminally mutated PLA(2) were carried out in the present work. Removal of N-terminal heptapeptide caused a complete loss of membrane-damaging activity, whilst the mutants with an extra Met before Asn-1 or… CONTINUE READING