Mutations in two Ku homologs define a DNA end-joining repair pathway in Saccharomyces cerevisiae.

@article{Milne1996MutationsIT,
  title={Mutations in two Ku homologs define a DNA end-joining repair pathway in Saccharomyces cerevisiae.},
  author={Georgina Milne and Shengfang Jin and Katie B. Shannon and David T. Weaver},
  journal={Molecular and cellular biology},
  year={1996},
  volume={16 8},
  pages={4189-98}
}
DNA double-strand break (DSB) repair in mammalian cells is dependent on the Ku DNA binding protein complex. However, the mechanism of Ku-mediated repair is not understood. We discovered a Saccharomyces cerevisiae gene (KU80) that is structurally similar to the 80-kDa mammalian Ku subunit. Ku8O associates with the product of the HDF1 gene, forming the major DNA end-binding complex of yeast cells. DNA end binding was absent in ku80delta, hdf1delta, or ku80delta hdf1delta strains. Antisera… CONTINUE READING