Mutations in the processing site of the precursor of ribulose-1,5-bisphosphate carboxylase/oxygenase small subunit: effects on import, processing, assembly and stability

@article{Levy1995MutationsIT,
  title={Mutations in the processing site of the precursor of ribulose-1,5-bisphosphate carboxylase/oxygenase small subunit: effects on import, processing, assembly and stability},
  author={Maggie Levy and Zach Adam},
  journal={Plant Molecular Biology},
  year={1995},
  volume={29},
  pages={53-61}
}
The small subunit (SSU) of Rubisco is synthesized in the cytosol in a precursor form. Upon import into the chloroplast, it is proteolytically processed at a Cys-Met bond to yield the mature form of the protein. To assess the importance of the Met residue for recognition and processing by the stromal peptidase, we substituted this residue with either Thr, Arg or Asp. The mutant precursor proteins were imported into isolated chloroplasts, and the products of the import reactions were analyzed… CONTINUE READING
BETA

Similar Papers

References

Publications referenced by this paper.
SHOWING 1-10 OF 41 REFERENCES

A mutation in the small subunit ofribulose-l,5bisphosphate carboxylase/oxygenase that reduces the rate of its incorporation into holoenzyme

Z Adam
  • Photosynthesis Res, 43:143-147
  • 1995
VIEW 2 EXCERPTS

The N-end rule.

  • Cold Spring Harbor symposia on quantitative biology
  • 1995
VIEW 1 EXCERPT