Mutations in the procaspase-3 dimer interface affect the activity of the zymogen.

@article{Pop2003MutationsIT,
  title={Mutations in the procaspase-3 dimer interface affect the activity of the zymogen.},
  author={Cristina Pop and Brett Feeney and Ashutosh Tripathy and A. Clay Clark},
  journal={Biochemistry},
  year={2003},
  volume={42 42},
  pages={
          12311-20
        }
}
The interface of the procaspase-3 dimer plays a critical role in zymogen maturation. We show that replacement of valine 266, the residue at the center of the procaspase-3 dimer interface, with glutamate resulted in an increase in enzyme activity of approximately 60-fold, representing a pseudoactivation of the procaspase. In contrast, substitution of V266 with histidine abolished the activity of the procaspase-3 as well as that of the mature caspase. While the mutations do not affect the dimeric… CONTINUE READING

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