Mutations in the pore region modify epithelial sodium channel gating by shear stress.

@article{Carattino2005MutationsIT,
  title={Mutations in the pore region modify epithelial sodium channel gating by shear stress.},
  author={Marcelo D Carattino and Shaohu Sheng and Thomas R Kleyman},
  journal={The Journal of biological chemistry},
  year={2005},
  volume={280 6},
  pages={4393-401}
}
Previous studies have shown that epithelial Na+ channels (ENaCs) are activated by laminar shear stress (LSS). ENaCs with a high intrinsic open probability because of a mutation (betaS518K) or covalent modification of an introduced Cys residue (alphaS580C) in the pre-second transmembrane domain (pre-M2) were not activated by LSS, suggesting that the pre-M2 region participates in conformational rearrangements during channel activation. We examined the role of the pore region of the alpha-subunit… CONTINUE READING