Mutations in the cyp51A gene and susceptibility to itraconazole in Aspergillus fumigatus serially isolated from a patient with lung aspergilloma.

@article{Chen2005MutationsIT,
  title={Mutations in the cyp51A gene and susceptibility to itraconazole in Aspergillus fumigatus serially isolated from a patient with lung aspergilloma.},
  author={Jian Chen and Houmin Li and Ruoyu Li and Dingfang Bu and Zhe Wan},
  journal={The Journal of antimicrobial chemotherapy},
  year={2005},
  volume={55 1},
  pages={31-7}
}
OBJECTIVES To monitor changes in itraconazole susceptibility of isolates from a patient undergoing treatment for pulmonary Aspergillus infection and relate these changes to genotypic/phenotypic alterations. METHODS Six Aspergillus fumigatus isolates were serially recovered from the patient. Itraconazole MICs were determined by Etest and NCCLS methodology. Growth characteristics and phenotype were monitored. Molecular analysis included random amplified polymorphic DNA (RAPD) assay and… CONTINUE READING