Mutations in the N-terminal region of RecA that disrupt the stability of free protein oligomers but not RecA-DNA complexes.

@article{Eldin2000MutationsIT,
  title={Mutations in the N-terminal region of RecA that disrupt the stability of free protein oligomers but not RecA-DNA complexes.},
  author={S S Eldin and Anthony L. Forget and Danielle M. Lindenmuth and Krystin M Logan and Kendall L. Knight},
  journal={Journal of molecular biology},
  year={2000},
  volume={299 1},
  pages={91-101}
}
We have introduced targeted mutations in two areas that make up part of the RecA subunit interface. In the RecA crystal structure, cross-subunit interactions are observed between the Lys6 and Asp139 side-chains, and between the Arg28 and Asn113 side-chains. Unexpectedly, we find that mutations at Lys6 and Arg28 impose sever defects on the oligomeric stability of free RecA protein, whereas mutations at Asn113 or Asp139 do not. However, Lys6 and Arg28 mutant proteins showed an apparent normal… CONTINUE READING

From This Paper

Topics from this paper.

Citations

Publications citing this paper.
Showing 1-10 of 12 extracted citations

Similar Papers

Loading similar papers…