Mutations in sigma factor that affect the temperature dependence of transcription from a promoter, but not from a mismatch bubble in double-stranded DNA.

@article{Aiyar1994MutationsIS,
  title={Mutations in sigma factor that affect the temperature dependence of transcription from a promoter, but not from a mismatch bubble in double-stranded DNA.},
  author={Sarah E. Aiyar and Y L Juang and J. D. Helmann and Pieter L Dehaseth},
  journal={Biochemistry},
  year={1994},
  volume={33 38},
  pages={11501-6}
}
Specificity of promoter utilization in bacterial RNA polymerases is imparted by a class of proteins referred to as sigma factors. Conserved region 2.3 of these proteins is thought to play a role in the strand separation process that occurs during the formation of an initiation-competent RNA polymerase-promoter complex. We have used a heterologous system consisting of Escherichia coli core RNA polymerase and Bacillus subtilis sigma A to probe the effects of amino acid substitutions in region 2.3… CONTINUE READING

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