Mutations in eIF5B Confer Thermosensitive and Pleiotropic Phenotypes via Translation Defects in Arabidopsis thaliana.

@article{Zhang2017MutationsIE,
  title={Mutations in eIF5B Confer Thermosensitive and Pleiotropic Phenotypes via Translation Defects in Arabidopsis thaliana.},
  author={Liyuan Zhang and Xinye Liu and Kishor Gaikwad and Xiaoxia Kou and Fei Wang and Xuejun Tian and Mingming Xin and Zhongfu Ni and Qixin Sun and Huiru Peng and Elizabeth Vierling},
  journal={The Plant cell},
  year={2017},
  volume={29 8},
  pages={1952-1969}
}
The conserved eukaryotic translation initiation factor 5B, eIF5B, is a GTPase that acts late in translation initiation. We found that an Arabidopsis thaliana mutant sensitive to hot temperatures 3 (hot3-1), which behaves as the wild type in the absence of stress but is unable to acclimate to high temperature, carries a missense mutation in the eIF5B1 gene (At1g76810), producing a temperature sensitive protein. A more severe, T-DNA insertion allele (hot3-2) causes pleiotropic developmental… CONTINUE READING