Mutations in a single signaling pathway allow cell growth in heavy water.

@article{Kampmeyer2020MutationsIA,
  title={Mutations in a single signaling pathway allow cell growth in heavy water.},
  author={Caroline Kampmeyer and Jens Vilstrup Johansen and Christian Holmberg and Magnus Karlson and Sarah K Gersing and Helo{\'i}sa Nunes Bordallo and Birthe B. Kragelund and Mathilde H. Lerche and Isabelle Jourdain and Jakob Rahr Winther and Rasmus Hartmann-Petersen},
  journal={ACS synthetic biology},
  year={2020}
}
Life is completely dependent on water. To analyze the role of water as a solvent in biology, we replaced water with heavy water (D2O), and investigated the biological effects by a wide range of techniques, using Schizosaccharomyces pombe as model organism. We show that high concentrations of D2O lead to altered glucose metabolism and growth retardation. After prolonged incubation in D2O, cells displayed gross morphological changes, thickened cell walls and aberrant cytoskeletal organization. By… 
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References

SHOWING 1-10 OF 90 REFERENCES
Microarray analyses of the metabolic responses of Saccharomyces cerevisiae to organic solvent dimethyl sulfoxide
TLDR
The results demonstrate that the application of microarray technology allows better interpretation of metabolic responses, and the information obtained will be useful for the construction of engineered yeast strains with better tolerance of organic solvents.
Spm1, a stress‐activated MAP kinase that regulates morphogenesis in S.pombe
TLDR
The results demonstrate the existence of a new MAP kinase pathway that regulates cell wall remodeling and cytokinesis in response to environmental stresses.
Global transcriptional responses of fission yeast to environmental stress.
TLDR
Transcriptional responses of the fission yeast Schizosaccharomyces pombe to various environmental stresses were explored and promoter motifs associated with some of the groups of coregulated genes were identified.
Hypersensitivity to heavy water: A new conditional phenotype
TLDR
The ds technique should be useful for producing conditional mutations in genes that are not amenable to the ts and cs approaches, and also for generating alternative conditional (ds) alleles in many other genes.
Ksg1, a homologue of the phosphoinositide‐dependent protein kinase 1, controls cell wall integrity in Schizosaccharomyces pombe
TLDR
Evidence is provided that ksg1 is a novel regulator of cell wall integrity in the fission yeast Schizosaccharomyces pombe and that Ksg1p acts in a pathway with Pck1p, possibly upstream and through direct interaction.
Redox control and oxidative stress in yeast cells.
Protein structure and function can be altered by reactive oxygen species produced either by cell metabolism or by external oxidants. Although catalases, superoxide dismutases and peroxidases
Stress-controlled transcription factors, stress-induced genes and stress tolerance in budding yeast.
  • F. Estruch
  • Biology, Medicine
    FEMS microbiology reviews
  • 2000
TLDR
The possibility of using genetic approaches has made the yeast Saccharomyces cerevisiae a compelling model to study stress response at a molecular level and this information can be used to isolate and characterise stress-related proteins in higher eukaryotes and to design strategies to increase stress resistance in organisms of industrial interest.
Regulation of Cell Wall Biogenesis in Saccharomyces cerevisiae: The Cell Wall Integrity Signaling Pathway
TLDR
This review article centers on CWI signaling in Saccharomyces cerevisiae through the cell cycle and in response to cell wall stress.
Increased expression of Hsp70 for resistance to deuterium oxide in a yeast mutant cell line.
TLDR
A mutant of yeast is isolated that grows well in D(2)O and the expression of Hsp70 was enhanced in the mutant, which might be useful for biosynthesis of D-labeled biomolecules.
Multiple layers of regulation influence cell integrity control by the PKC ortholog Pck2 in fission yeast
TLDR
A novel mechanism whereby TOR regulates PKC function at a translational level is unveiled, and TORC2 contributes to de novo Pck2 synthesis, which is essential to activate the CIP in response to cell wall damage or glucose exhaustion.
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