Mutations in Saccharomyces cerevisiae gene SIR2 can have differential effects on in vivo silencing phenotypes and in vitro histone deacetylation activity.

@article{Armstrong2002MutationsIS,
  title={Mutations in Saccharomyces cerevisiae gene SIR2 can have differential effects on in vivo silencing phenotypes and in vitro histone deacetylation activity.},
  author={Christopher M. Armstrong and Matt Kaeberlein and Shin Ichiro Imai and Leonard P. Guarente},
  journal={Molecular biology of the cell},
  year={2002},
  volume={13 4},
  pages={1427-38}
}
The yeast SIR2 gene and many of its homologs have been identified as NAD(+)-dependent histone deacetylases. To get a broader view of the relationship between the histone deacetylase activity of Sir2p and its in vivo functions we have mutated eight highly conserved residues in the core domain of SIR2. These mutations have a range of effects on the ability of Sir2p to deacetylate histones in vitro and to silence genes at the telomeres and HM loci. Interestingly, there is not a direct correlation… CONTINUE READING

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A phylogenetically conserved NAD+-dependent protein deacetylase activity in the Sir2 protein family.

Proceedings of the National Academy of Sciences of the United States of America • 2000
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