Mutations conferring aminoglycoside and spectinomycin resistance in Borrelia burgdorferi.

@article{Criswell2006MutationsCA,
  title={Mutations conferring aminoglycoside and spectinomycin resistance in Borrelia burgdorferi.},
  author={Daniel C. Criswell and Virginia L Tobiason and J Stephen Lodmell and D. Scott Samuels},
  journal={Antimicrobial agents and chemotherapy},
  year={2006},
  volume={50 2},
  pages={445-52}
}
We have isolated and characterized in vitro mutants of the Lyme disease agent Borrelia burgdorferi that are resistant to spectinomycin, kanamycin, gentamicin, or streptomycin, antibiotics that target the small subunit of the ribosome. 16S rRNA mutations A1185G and C1186U, homologous to Escherichia coli nucleotides A1191 and C1192, conferred >2,200-fold and 1,300-fold resistance to spectinomycin, respectively. A 16S rRNA A1402G mutation, homologous to E. coli A1408, conferred >90-fold resistance… CONTINUE READING