Mutations altering the cleavage specificity of a homing endonuclease.

@article{Seligman2002MutationsAT,
  title={Mutations altering the cleavage specificity of a homing endonuclease.},
  author={Lenny M. Seligman and Karen M. Chisholm and B. I. Gilman And C. S. Peirce Jean - Marie Chevalier and Meggen S. Chadsey and Samuel T Edwards and Jeremiah H Savage and Adeline L Veillet},
  journal={Nucleic acids research},
  year={2002},
  volume={30 17},
  pages={3870-9}
}
The homing endonuclease I-CreI recognizes and cleaves a particular 22 bp DNA sequence. The crystal structure of I-CreI bound to homing site DNA has previously been determined, leading to a number of predictions about specific protein-DNA contacts. We test these predictions by analyzing a set of endonuclease mutants and a complementary set of homing site mutants. We find evidence that all structurally predicted I-CreI/DNA contacts contribute to DNA recognition and show that these contacts differ… CONTINUE READING

From This Paper

Figures, tables, and topics from this paper.

Citations

Publications citing this paper.
Showing 1-10 of 47 extracted citations

References

Publications referenced by this paper.
Showing 1-10 of 39 references

Similar Papers

Loading similar papers…