Mutations activating the yeast eIF-2 alpha kinase GCN2: isolation of alleles altering the domain related to histidyl-tRNA synthetases.

@article{Ramrez1992MutationsAT,
  title={Mutations activating the yeast eIF-2 alpha kinase GCN2: isolation of alleles altering the domain related to histidyl-tRNA synthetases.},
  author={Melissa A Ram{\'i}rez and Ronald C Wek and Carlos R. V{\'a}zquez de Aldana and Belinda M. Jackson and Barbie Freeman and Alan G Hinnebusch},
  journal={Molecular and cellular biology},
  year={1992},
  volume={12 12},
  pages={5801-15}
}
The protein kinase GCN2 stimulates expression of the yeast transcriptional activator GCN4 at the translational level by phosphorylating the alpha subunit of translation initiation factor 2 (eIF-2 alpha) in amino acid-starved cells. Phosphorylation of eIF-2 alpha reduces its activity, allowing ribosomes to bypass short open reading frames present in the GCN4 mRNA leader and initiate translation at the GCN4 start codon. We describe here 17 dominant GCN2 mutations that lead to derepression of GCN4… CONTINUE READING

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