Mutational mapping of kinetic and pharmacological properties of a human cardiac cAMP phosphodiesterase.

@article{Pillai1994MutationalMO,
  title={Mutational mapping of kinetic and pharmacological properties of a human cardiac cAMP phosphodiesterase.},
  author={Ramesh Kumar Gopala Pillai and S F Staub and John Colicelli},
  journal={The Journal of biological chemistry},
  year={1994},
  volume={269 48},
  pages={30676-81}
}
We have created a series of deletion mutants of a human cardiac cAMP phosphodiesterase in order to define sequences necessary for function and to identify residues required for inhibition by cGMP and by the drugs milrinone and trequinsin. These truncated constructs were expressed in yeast cells, and their biochemical properties were analyzed. The mutations define an amino acid sequence that is essential for function. Among the active constructs, there was considerable variability in the level… CONTINUE READING