Mutational cloning in Streptomyces and the isolation of antibiotic production genes.

@article{Chater1983MutationalCI,
  title={Mutational cloning in Streptomyces and the isolation of antibiotic production genes.},
  author={Keith F. Chater and Celia J. Bruton},
  journal={Gene},
  year={1983},
  volume={26 1},
  pages={67-78}
}
An attachment site-deleted derivative, phi C31KC400, of the Streptomyces temperate phage phi C31 was used to clone fragments of the genetic determinants (mmy) for the biosynthesis of an antibiotic, methylenomycin A. The vector carries a cloned viomycin resistance gene (vph), and can transduce a recipient to viomycin resistance when DNA sequences are common to the phage and the recipient: the phage integrates into the recipient's genome through a Campbell type of recombination at the site of the… CONTINUE READING

From This Paper

Topics from this paper.
38 Citations
0 References
Similar Papers

Citations

Publications citing this paper.
Showing 1-10 of 38 extracted citations

Similar Papers

Loading similar papers…