Mutational biosynthesis of novel rapamycins by a strain of Streptomyces hygroscopicus NRRL 5491 disrupted in rapL, encoding a putative lysine cyclodeaminase.

@article{Khaw1998MutationalBO,
  title={Mutational biosynthesis of novel rapamycins by a strain of Streptomyces hygroscopicus NRRL 5491 disrupted in rapL, encoding a putative lysine cyclodeaminase.},
  author={L E Khaw and G{\"u}nter A. B{\"o}hm and Su M. Metcalfe and James C Staunton and Peter Francis Leadlay},
  journal={Journal of bacteriology},
  year={1998},
  volume={180 4},
  pages={809-14}
}
The gene rapL lies within the region of the Streptomyces hygroscopicus chromosome which contains the biosynthetic gene cluster for the immunosuppressant rapamycin. Introduction of a frameshift mutation into rapL by phiC31 phage-mediated gene replacement gave rise to a mutant which did not produce significant amounts of rapamycin. Growth of this rapL mutant on media containing added L-pipecolate restored wild-type levels of rapamycin production, consistent with a proposal that rapL encodes a… CONTINUE READING

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References

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Showing 1-10 of 26 references

Rapamycin, FK506, and ascomycin-related compounds, p. 497–520

  • K. A. Reynolds, A. L. Demain
  • Drugs and pharmaceutical sciences series,
  • 1997

The nature of the starter unit for the rapamycin polyketide synthase

  • P.A.S. Lowden, G. A. Böhm, J. Staunton, P. F. Leadlay
  • 1996

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