Mutational and spectroscopic studies of the significance of the active site glutamine to metal ion specificity in superoxide dismutase.

@article{Schwartz2000MutationalAS,
  title={Mutational and spectroscopic studies of the significance of the active site glutamine to metal ion specificity in superoxide dismutase.},
  author={Amy L. Schwartz and Emine Yikilmaz and Carrie K Vance and S Vathyam and Ronald L. Koder and Anne Frances Miller},
  journal={Journal of inorganic biochemistry},
  year={2000},
  volume={80 3-4},
  pages={247-56}
}
We are addressing the puzzling metal ion specificity of Fe- and Mn-containing superoxide dismutases (SODs) [see C.K.Vance, A.-F. Miller. J. Am. Chem. Soc. 120(3) (1998) 461-467]. Here, we test the significance to activity and active site integrity of the Gln side chain at the center of the active site hydrogen bond network. We have generated a mutant of MnSOD with the active site Gln in the location characteristic of Fe-specific SODs. The active site is similar to that of MnSOD when Mn2+, Fe3… CONTINUE READING
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References

Publications referenced by this paper.
Showing 1-10 of 14 references

37 (1998) 4731–4739

  • M. S. Lah, M. M. Dixon, K. A. Pattridge, W. C. Stallings, J. A. Fee
  • M.L. Ludwig, Biochemistry 34 (1995) 1646–1660…
  • 1985
Highly Influential
6 Excerpts

Biochemistry 36 (1997) 4916–4924

  • D. L. Sorkin, A.-F. Miller
  • Ohmori, T. Matsumoto, Biochem. Mol. Biol. Int. 36
  • 1995
Highly Influential
4 Excerpts

Chem

  • C. K. Vance, A.-F. Miller, J. Am
  • Soc. 120
  • 1998

M

  • K. Bunting, J. B. Cooper, M. O. Badasso, I. J. Tickle
  • Newton, S.P. [42] G.C. Levy, R.L. Lichter, in…
  • 1998
1 Excerpt

Biochemistry 30 (1991) 3210–3216

  • T. Hunter, K. Ikebukuro, W. H. Bannister, J. V. Bannister, G. J. Hunter
  • Biochemistry 36
  • 1997
1 Excerpt

Biomol

  • C. L. Fisher, J.-L. Chen, J. Li, D. Bashford, L. Noodleman, J. Phys
  • NMR 6 (1995) 277–293. Chem. 100
  • 1996
1 Excerpt

Eur

  • Y. Guan, M. J. Hickey, +5 authors K. Murayama
  • J. Biochem. Lepock, D. O’Connor, Y. Hsieh, H.S…
  • 1992
1 Excerpt

Evol

  • M. W. Smith, R. F. Doolittle, J. Mol
  • 34 (1992) 175–184.
  • 1990
1 Excerpt

Biol

  • M. W. Parker, C.C.F. Blake, J. Mol
  • 199
  • 1988
1 Excerpt

FEBS Lett

  • M. W. Parker, C.C.F. Blake
  • 229
  • 1988
1 Excerpt

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