Mutational analysis reveals that all tailoring region genes are required for production of polyketide antibiotic mupirocin by pseudomonas fluorescens: pseudomonic acid B biosynthesis precedes pseudomonic acid A.

@article{Hothersall2007MutationalAR,
  title={Mutational analysis reveals that all tailoring region genes are required for production of polyketide antibiotic mupirocin by pseudomonas fluorescens: pseudomonic acid B biosynthesis precedes pseudomonic acid A.},
  author={J. Daniel Hothersall and Ji'en Wu and Ayesha S. Rahman and Jennifer A Shields and James A Haddock and Nicola F. Johnson and Sian M Cooper and Elton R. Stephens and Russell J Cox and John Crosby and Christine L Willis and Thomas J Simpson and Christopher M. Thomas},
  journal={The Journal of biological chemistry},
  year={2007},
  volume={282 21},
  pages={15451-61}
}
The Pseudomonas fluorescens mupirocin biosynthetic cluster encodes six proteins involved in polyketide biosynthesis and 26 single polypeptides proposed to perform largely tailoring functions. In-frame deletions in the tailoring open reading frames demonstrated that all are required for mupirocin production. A bidirectional promoter region was identified between mupF, which runs counter to other open reading frames and its immediate neighbor macpC, implying the 74-kb cluster consists of two… CONTINUE READING