Mutational analysis of yeast vacuolar H(+)-ATPase.

  title={Mutational analysis of yeast vacuolar H(+)-ATPase.},
  author={Takato Noumi and Constantino Beltran and H. Roice Nelson and Nathan Alfred Nelson},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  volume={88 5},
Yeast mutants in which genes encoding subunits of the vacuolar H(+)-ATPase were interrupted were assayed for their vacuolar ATPase and proton-uptake activities. The vacuoles from the mutants lacking subunits A (72 kDa), B (57 kDa), or c (proteolipid, 16 kDa) were completely inactive in these reactions. Immunological studies revealed that in the absence of each one of those subunits the catalytic sector was not assembled. Labeling with N,N'-[14C]dicyclohexylcarbodiimide showed the presence of… CONTINUE READING
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