Mutational analysis of the input domain of the VirA protein of Agrobacterium tumefaciens.

@article{Doty1996MutationalAO,
  title={Mutational analysis of the input domain of the VirA protein of Agrobacterium tumefaciens.},
  author={Sharon Lafferty Doty and Michael Chiwei Yu and Jessica Lundin and Joe Don Heath and Eugene W. Nester},
  journal={Journal of bacteriology},
  year={1996},
  volume={178 4},
  pages={961-70}
}
The transmembrane sensor protein VirA activates VirG in response to high levels of acetosyringone (AS). In order to respond to low levels of AS, VirA requires the periplasmic sugar-binding protein ChvE and monosaccharides released from plant wound sites. To better understand how VirA senses these inducers, the C58 virA gene was randomly mutagenized, and 14 mutants defective in vir gene induction and containing mutations which mapped to the input domain of VirA were isolated. Six mutants had… CONTINUE READING

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To better understand how VirA senses these inducers , the C58 virA gene was randomly mutagenized , and 14 mutants defective in vir gene induction and containing mutations which mapped to the input domain of VirA were isolated .
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