Mutational analysis of the group A streptococcal operon encoding streptolysin S and its virulence role in invasive infection
@article{Datta2005MutationalAO, title={Mutational analysis of the group A streptococcal operon encoding streptolysin S and its virulence role in invasive infection}, author={Vivekananda Datta and Sandra M Myskowski and Laura A Kwinn and Daniel N Chiem and Nissi Varki and Rita Kansal and Malak Kotb and Victor Nizet}, journal={Molecular Microbiology}, year={2005}, volume={56} }
The pathogen group A Streptococcus (GAS) produces a wide spectrum of infections including necrotizing fasciitis (NF). Streptolysin S (SLS) produces the hallmark β‐haemolytic phenotype produced by GAS. The nine‐gene GAS locus (sagA–sagI) resembling a bacteriocin biosynthetic operon is necessary and sufficient for SLS production. Using precise, in‐frame allelic exchange mutagenesis and single‐gene complementation, we show sagA, sagB, sagC, sagD, sagE, sagF and sagG are each individually…
170 Citations
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