Mutational analysis of the PRP4 protein of Saccharomyces cerevisiae suggests domain structure and snRNP interactions.

@article{Hu1994MutationalAO,
  title={Mutational analysis of the PRP4 protein of Saccharomyces cerevisiae suggests domain structure and snRNP interactions.},
  author={James W. Hu and Yanzhang Xu and Keith Schappert and Tim Harrington and Anan Wang and Rafael Utsch Braga and Jeremy Mogridge and James D. Friesen},
  journal={Nucleic acids research},
  year={1994},
  volume={22 9},
  pages={1724-34}
}
The PRP4 protein of Saccharomyces cerevisiae is an essential part of the U4/U6 snRNP, a component of the mRNA splicing apparatus. As an approach to the determination of structure-function relationships in the PRP4 protein, we have isolated more than fifty new alleles of the PRP4 gene through random and site-directed mutagenesis, and have analyzed the phenotypes of many of them. Twelve of the fourteen single-point mutations that give rise to temperature-sensitive (ts) or null phenotypes are… CONTINUE READING

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