Mutational analysis of polynucleotide phosphorylase from Escherichia coli.

  title={Mutational analysis of polynucleotide phosphorylase from Escherichia coli.},
  author={Anne Jarrige and Dominique Br{\'e}chemier-Baey and Nathalie L. Mathy and Oph{\'e}lie Duch{\'e} and Claude Portier},
  journal={Journal of molecular biology},
  volume={321 3},
Polynucleotide phosphorylase (PNPase), a homotrimeric exoribonuclease present in bacteria, is involved in mRNA degradation. In Escherichia coli, expression of this enzyme is autocontrolled at the translational level. We introduced about 30 mutations in the pnp gene by site-directed mutagenesis, most of them in phylogenetically conserved residues, and determined their effects on the three catalytic activities of PNPase, phosphorolysis, polymerisation and phosphate exchange, as well as on the… CONTINUE READING
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