Mutational analysis of microbial hydroxycinnamoyl-CoA hydratase-lyase (HCHL) towards enhancement of binding affinity: A computational approach.

@article{Kumar2017MutationalAO,
  title={Mutational analysis of microbial hydroxycinnamoyl-CoA hydratase-lyase (HCHL) towards enhancement of binding affinity: A computational approach.},
  author={Pravin Kumar and S. Ghosh Sachan and R. Poddar},
  journal={Journal of molecular graphics & modelling},
  year={2017},
  volume={77},
  pages={
          94-105
        }
}
  • Pravin Kumar, S. Ghosh Sachan, R. Poddar
  • Published 2017
  • Chemistry, Medicine
  • Journal of molecular graphics & modelling
  • Improving the industrial enzyme for better yield of the product is important and a challenging task. One of such important industrial enzymes is microbial Hydroxycinnamoyl-CoA hydratase-lyase (HCHL). It converts feruloyl-CoA to vanillin. We place our efforts towards the improvement of its catalytic activity with comprehensive computational investigation. Catalytic core of the HCHL was explored with molecular modeling and docking approaches. Site-directed mutations were introduced in the… CONTINUE READING
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